Fractionation, Collection, and Purification
The challenges for collecting liquids after a chromatographic separation have become wide ranged. LEAP is one of few companies that offer practically the whole range of needs. From large bed large volume purification processed all the way to micro or nano liter droplets onto target plates. A very unique fraction collector that gains popularity is a sprayer that sprays the outflow of normal or micro flow onto a target plate for subsequent analysis by MALDI-MS, FTIR or Ramen.
The two most popular systems are based on the CTC PAL platform. One is called “CollectPAL” which serves the needs where fractions are collected mostly on a time interval basis and the “Purific System” that is used for mass determined fractionation after a high flow HPLC run.
CollectPAL
Radioactive labeled samples in the profiling area of ADME/TOX are fractionated by a simple Collection-PAL. Several features of this equipment serve to prevent un-controlled evaporation but also better security when handling radioactive material. The ability to keep microtiter plates covered and cooled. But the key request that customers have is to be able to collect 100% of the column eluent. A proprietary syringe and plunger control concept makes it possible that during the movement forward to the next well the eluent can temporarily be held in the syringe. Reproducible volumes in each fraction and up to 24 plate capacity 96- or 384-well plates.
An output file lists the exact time when each fraction collection started. A precise retention time match is therefore possible. All is powered by LEAP Shell 3.
Other application where samples are not labeled can be handles either based on retention times or start and end triggers from the chromatographic system.
Purific System
In so called “production” situations a large bed CTC PAL with dual syringe system makes the sample injection onto the semi-prep LC and collects with the second head the fractions into tubes. The power resides within its software called “Peak Harvester” that manages the time of collection and tracks the fraction’s association with the samples easily visible on the PC Screen for the operator. A high capacity of fraction tubes and an “open access” approach make this system suitable for replacing aging older systems. If mass-driven on-line or mass driven off-line we help you to evaluate your best option.
Nano-LC MALDI Prep
In case the resolution power of nano-LC or capillary columns is needed we can offer a package with the Chorus 220 no-split nano flow pump, Nano PAL and the PAL MALDI Kit. This package is being controlled by LEAP Shell 3 software and only requires a hardware signal for integration with an LC or MS data system.
LC-T (Spraying FC).
Originally exclusively used for the sample prep work in the polymer industry (formulation) where FTIR used to be the predominant way of identifying impurities and ingredients. The outflow of a chromatographic separation (HPLC or GPC) is getting into the LC-T’s heated spraying nozzle and is sprayed as a narrow (1-10mm) trace onto a Germanium disc or MALDI plate. For MALDI a matrix co-elution option is available.
